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      1  Cover

      2  An Introduction toMolecular Biotechnology

      3  Copyright

      4  Abbreviations

      5  Part I: Fundamentals of Cellular and Molecular Biology 1 The Cell as the Basic Unit of Life References Further Reading 2 Structure and Function of Cellular Macromolecules 2.1 Structure and Function of Sugars 2.2 Structure of Membrane Lipids 2.3 Structure and Function of Proteins 2.4 Structure of Nucleotides and Nucleic Acids (DNA and RNA) References Further Reading 3 Structure and Functions of a Cell 3.1 Structure of a Eukaryotic Cell 3.2 Structure of Bacteria 3.3 Structure of Viruses 3.4 Differentiation of Cells 3.5 Cell Death References Further Reading 4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) 4.1 Genomes, Chromosomes, and Replication 4.2 Transcription: From Gene to Protein 4.3 Protein Biosynthesis (Translation) Further Reading 5 Distributing Proteins in the Cell (Protein Sorting) 5.1 Import and Export of Proteins via the Nuclear Pore 5.2 Import of Proteins in Mitochondria, Chloroplasts, and Peroxisomes 5.3 Protein Transport into the Endoplasmic Reticulum 5.4 Vesicle Transport from the ER via the Golgi Apparatus to the Cytoplasmic Membrane References Further Reading 6 Evolution and Diversity of Organisms 6.1 Prokaryotes 6.2 Eukaryotes References Further Reading

      6  Part II: Standard Methods in Molecular Biotechnology 7 Isolation and Purification of Proteins 7.1 Introduction 7.2 Producing a Protein Extract 7.3 Gel Electrophoretic Separation Methods 7.4 Methods of Protein Precipitation 7.5 Column Chromatography Methods 7.6 Examples Further Reading 8 Mass Spectrometry and Applications in Proteomics and Microbial Identification 8.1 Principles of ESI and MALDI Mass Spectrometry 8.2 Instrumental Setup 8.3 Intact Protein Analysis 8.4 Protein and Proteome Quantification 8.5 Protein–Protein Interaction Analysis 8.6 Analysis of Posttranslational Modifications 8.7 Microbial Identification and Resistance Detection References 9 Isolation of DNA and RNA 9.1 Introduction 9.2 DNA Isolation 9.3 RNA Isolation Reference 10 Chromatography and Electrophoresis of Nucleic Acids 10.1 Introduction 10.2 Chromatographic Separation of Nucleic Acids 10.3 Electrophoresis Further Reading 11 Hybridization of Nucleic Acids 11.1 Significance of Base Pairing 11.2 Experimental Hybridization: Kinetic and Thermodynamic